Cyclic AMP EIA Kit

Direct EIA kit used for quantitative measuring cAMP present in Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Heparin Plasma, Tissue Culture Media samples from all species

Adenosine-3',5'-cyclic monophosphate (cyclic AMP) is a second messenger that plays an important role in intracellular regulation. Specifically, it functions as a mediator of activity of several key hormones including epinephrine, glucagon, and ACTH (1-4). Adenylate cyclase is activated by the hormones glucagon and adrenaline and by G protein. Liver adenylate cyclase responds more strongly to glucagon, and muscle adenylate cyclase responds more strongly to adrenaline. cAMP decomposition into AMP is catalyzed by the enzyme phosphodiesterase. In the Human Metabolome Database there are 166 metabolic enzymes listed that convert cAMP (5). Other biological actions of cAMP include regulation of innate immune functioning (6), axon regeneration (7), cancer (8), and inflammation (9).

The kit is transported on ice packs (+4ºC). Upon receipt store at 4ºC. Non-hazardous item according to both ADR and UN regulations.

• Assay type: Direct EIA (Enzyme Immunoassay)
• Species reactivity: Species Independent
• Sample types: Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Heparin Plasma, Tissue Culture Media
• Sensitivity: 0.64 pmol/ml
• Assay range: Regular: 0.617 - 150 pmol/ml. Acetylated: 0.156 - 10 pmol/ml
• Incubation t 2.5 Hours
• Number of samples per kit: 39 samples in duplicate
• Method of detection: Colorimetric Assay

For research use only. Not to be used for human or animal treatment or diagnostics.