Cyclic GMP EIA Kit

Direct EIA kit used for quantitative measuring cGMP present in Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Tissue Culture Media samples from all species

• Guanosine 3', 5'-cyclic monophosphate (cyclic GMP
• cGMP) is a critical and multifunctional second messenger present at levels typically 10-100 fold lower than cAMP in most tissues. Intracellular cGMP is formed by the action of the enzyme guanylate cyclase on GTP and degraded through phosphodiesterase hydrolysis (1-3). Guanylate cyclases (GC) are either soluble or membrane bound (3,4). Soluble GCs are nitric oxide responsive, whereas membrane bound GCs respond to hormones such as acetylcholine, insulin and oxytocin. Other chemicals like serotonin and histamine also cause an increase in cGMP levels (5,6). Cyclic GMP regulates cellular composition through cGMP-dependent kinase, cGMP-dependent ion channels or transporters, and through its hydrolytic degradation by phosphodiesterase (1,7)

The kit is transported on ice packs (+4ºC). Upon receipt store at 4ºC. Non-hazardous item according to both ADR and UN regulations.

• Assay type: Direct EIA (Enzyme Immunoassay)
• Species reactivity: Species Independent
• Sample types: Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Tissue Culture Media
• Sensitivity: 0.28 pmol/ml
• Assay range: Regular: 0.5 - 32 pmol/ml. Acetylated: 0.3125 - 20 pmol/ml
• Incubation t
• Number of samples per kit: 39 samples in duplicate
• Method of detection: Colorimetric Assay

For research use only. Not to be used for human or animal treatment or diagnostics.