SARS-CoV-2 (COVID-19) NSP15 Antibody

Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP15 is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

• SARS-CoV-2 (COVID-19) NSP15 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Research area: Infectious Disease, COVID-19
• Host species: Rabbit
• Species reactivity: Virus
• Homology: Predicted reactivity based on immunogen sequence: SARS-CoV NSP15 protein: (100%).
• Immunogen: Anti-SARS-CoV-2 (COVID-19) NSP15 antibody (9269) was raised against a peptide corresponding to 15 amino acids near the carboxyl terminus of SARS-CoV-2 (COVID-19) NSP15 protein.
• The immunogen is located within the last 50 amino acids of the SARS-CoV-2 (COVID-19) NSP15 protein.
• Tested Applications: E, WB
• Applications: WB: 0.5-1 μ
• g/mL
• Antibody validated: SARS-CoV-2 (COVID-19) NSP15 antibody can detect 2 ng of free peptide at 1 μ
• g/mL in ELISA. It can detect SARS-CoV-2 NSP15 recombinant protein by ELISA and WB. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: N/A
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: SARS-CoV-2 (COVID-19) NSP15 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: SARS-CoV-2 (COVID-19) NSP15 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: NSP15
• Accession #: 6VWW_B
• Protein GI #: 1851775710
• NCBI gene ID#: 43740578
• NCBI official full name: ORF1a polyprotein
• ORF1ab polyprotein
• NCBI organism: Severe acute respiratory syndrome coronavirus 2
• SWISSPROT #: P0DTC1

Optimal dilutions for each application to be determined by the researcher.