PROM1 Antibody

PROM1 is a pentaspan transmembrane glycoprotein that localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation (1). Mutations in this gene have been shown to result in retinitis pigmentosa (2). Expression of this gene is also associated with several types of cancer (3).

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

• PROM1 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

• Research area: Stem Cell, Cancer
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: N/A
• Immunogen: PROM1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human PROM1.
• The immunogen is located within the last 50 amino acids of PROM1.
• Tested Applications: E, WB, ICC, IF
• Applications: PROM1 antibody can be used for detection of PROM1 by Western blot at 1 - 2 μ
• g/ml. Antibody can also be used for Immunocytochemistry at 2.5 μ
• g/mL. For Immunoflorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: PROM1 antibody is human, mouse and rat reactive. Multiple isoforms of PROM1 are known to exist.
• Positive control 1: Cat. No. 1205 - Jurkat Cell Lysate
• Positive control 2: Cat. No. 17-005 - Jurkat Cell Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 95 kDa
• Observed: 97 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: PROM1 antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: PROM1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: PROM1
• Accession #: NP_006008
• Protein GI #: 5174387
• NCBI gene ID#: 8842
• NCBI official full name: Prominin 1
• NCBI organism: homo sapiens
• SWISSPROT #: O43490

Optimal dilutions for each application to be determined by the researcher.