PRICKLE1 Antibody

PRICKLE1 Antibody: PRICKLE1, also known as RILP or EPM1B, is a Disheveled-associated protein that serves as a nuclear translocation receptor for REST/NRSF and REST4 (1, 2). It contains three N-terminal LIM domains and three C-terminal nuclear localization signals. It localizes to the cytoplasm, as well as to the nuclear membrane and expressed at higher levels in placenta. PRICKLE1 is a negative regulator of the Wnt / beta-catenin signaling pathway and is a putative tumor suppressor in human HCCs. Defects in the gene encoding PRICKLE1 are associated with autosomal recessive progressive myoclonic epilepsy.

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

• PRICKLE1 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Research area: Cancer, Cell Cycle
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: N/A
• Immunogen: PRICKLE1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PRICKLE1.
• The immunogen is located within the last 50 amino acids of PRICKLE1.
• Tested Applications: E, WB, IF
• Applications: PRICKLE1 antibody can be used for detection of PRICKLE1 by Western blot at 1 μ
• g/mL. Antibody can also be used for immunofluorescence starting at 20 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: Multiple isoforms of PRICKLE1 are known to exist. PRICKLE1 antibody is predicted to not cross-react with other PRICKLE protein family members.
• Positive control 1: Cat. No. 1310 - Human Bladder Tissue Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 46, 91 kDa
• Observed: 52, 90 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: PRICKLE1 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: PRICKLE1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: PRICKLE1
• Accession #: NP_694571
• Protein GI #: 222136684
• NCBI gene ID#: 144165
• NCBI official full name: prickle homolog 1
• NCBI organism: Homo sapiens
• SWISSPROT #: Q96MT3

Optimal dilutions for each application to be determined by the researcher.