N-RAS Antibody

N-RAS Antibody: Activating mutations and overexpression of classical Ras subfamily members (N-RAS, H-RAS, and K-RAS) have been widely investigated as key events in the development of human cancers. The N-RAS protein shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. N-RAS, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

• N-RAS antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

• Research area: Cancer, Cell Cycle
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: Predicted species reactivity based on immunogen sequence: Pig: (100%), Guinea pig: (93%), Chicken: (80%)
• Immunogen: N-RAS antibody was raised against a 15 amino acid peptide near the carboxy terminus of human N-RAS.
• The immunogen is located within the last 50 amino acids of N-RAS.
• Tested Applications: E, WB
• Applications: N-RAS antibody can be used for detection of N-RAS by Western blot at 1 and 2 μ
• g/mL.
• Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
• Specificity: N-RAS antibody is predicted to not cross-react with H-RAS
• Positive control 1: Cat. No. 1202 – A431 Cell Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 22 kDa
• Observed: 22 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: N-RAS Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: N-RAS Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: NRAS
• Accession #: NP_002515
• Protein GI #: 4505451
• NCBI gene ID#: 4893
• NCBI official full name: neuroblastoma RAS viral (v-ras) oncogene homolog
• NCBI organism: homo sapiens
• SWISSPROT #: P01111

Optimal dilutions for each application to be determined by the researcher.