MERS S1 Recombinant Protein

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

• Tested Applications: E, WB
• Applications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 83.1 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Predicted Molecular Weight: 83.1 kDa
• Physical state: Lyophilized
• Buffer: PBS, pH7.4
• Concentration: N/A
• NCBI official symbol: N/A
• Accession #: N/A
• Protein GI: N/A
• NCBI gene ID#: N/A
• NCBI official full name: N/A
• NCBI organism: N/A
• Peptide sequence: N/A
• SWISSPROT #: N/A
• Background Reference 1: Wan Y, et al. J Virol. 2020. pii: JVI.00127-20.
• Background Reference 2: Benvenuto D, et al. J Med Virol. 2020.
• Background Reference 3: Chang CY, et al. AMB Express. 2020. 10(1):20.
• Background Reference 4: N/A
• Background Reference 5: N/A
• Source: HEK293 cells
• Species: MERS
• By Source: Human Cells
• By Species: Other
• Fusion tag: His Tag
• Sequence: Tyr 18 - Arg 751
• Biology activity: N/A
• Purity: >95% as determined by SDS-PAGE.

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