HVEM / TNFRSF14 Recombinant Protein

Herpesvirus entry mediator (HVEM) is also known as TNFRSF14, TR2 (TNF receptorlike molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LTβR, the receptor for lymphotoxin αβ heterotrimer. The role of the HVEM LIGHT /LTβ receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.

Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

• Tested Applications: E, WB
• Applications: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 44.4 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Predicted Molecular Weight: 44.4 kDa
• Physical state: Lyophilized
• Buffer: Tris with Glycine, Arginine and NaCl, pH7.5
• Concentration: N/A
• NCBI official symbol: TNFRSF14
• Accession #: NP_003811.2
• Protein GI: N/A
• NCBI gene ID#: 8764
• NCBI official full name: TNF receptor superfamily member 14
• NCBI organism: Homo sapiens
• Peptide sequence: N/A
• SWISSPROT #: Q92956
• Background Reference 1: Hsu, H. et al., 1997, J. Biol. Chem. 272:13471.
• Background Reference 2: Mauri, D.N. et al., 1998, Immunity 8:21.
• Background Reference 3: Montgomery, R.I. et al., 1996, Cell 87:427.
• Background Reference 4: Cheung,T.C.et al., 2005, Proc.Natl.Acad.Sci.USA102:13218-23.
• Background Reference 5: N/A
• Source: HEK293 cells
• Species: Human
• By Source: Human Cells
• By Species: Human
• Fusion tag: Mouse IgG Fc Tag
• Sequence: Leu 39 - Val 202
• Biology activity: N/A
• Purity: >95% as determined by SDS-PAGE.

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