Monkeypox Virus Real Time PCR Kit

Ask for price

Lorem ipsum asdokfopdskaf
Monkeypox Virus  Real Time PCR Kit
Monkeypox Virus Real Time PCR Kit

Catalog number: / Dimensione: / Prezzo: EUR

Disponibilità: In ordine Numero di catalogo: ZD-0076-02 Fornitore: Gentaur Genprice Taglia: 25 tests/kit

Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

The principle of the real-time detection is based on the fluorogenic 5’nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities in real-time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification.

All reagents should be stored at -20°C. Storage at +4°C is not recommended. All reagents can be used until the expiration date indicated on the kit label. Repeated thawing and freezing (> 3x) should be avoided, as this may reduce the sensitivity of the assay. Cool all reagents during the working steps. Reaction Mix should be stored in the dark.

Analysis sensitivity depends on the sample volume, elution volume, nucleic acid extraction methods and other factors. If you use the DNA extraction buffer in the kit, the analysis sensitivity is the same as it declares. However, when the sample volume is dozens or even hundreds of times greater than elution volume by some concentrating method, it can be much higher.

  • For use with: ABI Prism® 7000/7300/7500/7900/Step One Plus
  • iCycler iQ™ 4/iQ™ 5
  • Smart Cycle II
  • Bio-Rad CFX 96
  • Rotor Gene™ 6000
  • Mx3000P/3005P
  • MJ-Option2/Chromo4
  • LightCycler®480 Instrument

0 recensione per Monkeypox Virus Real Time PCR Kit

Aggiungi una recensione


☆ ☆ ☆ ☆ ☆  (0)

Prigrow X Series Medium for T9305

Prigrow X Series Medium per T9305

Prigrow X Series Medium per T9305 la coltura di cellule di mammifero è composta da formulazioni specifiche di alta qualità per la crescita ottimale di diversi tipi di cellule primarie. Forma: soluzione Torbidità: chiaro Solo per uso di ricerca e non per applicazioni terapeutiche o diagnostiche. I prodotti abm sono destinati esclusivamente a scopi di [...]
Puntali con filtro PCR

Puntali con filtro PCR

Disponibile!Trovare il giusto puntale per pipettePuntali con filtro PCR (alta qualità) I nostri puntali filtranti per PCR sono progettati per adattarsi a quasi tutti i tipi di pipette. Sono prodotti con materiali di altissima qualità in un ambiente sterile, assicurando che siano privi di contaminanti che possono influenzare i tuoi risultati. Non tutte le punte [...]

LEPU Test Kit ELISA Anticorpi Neutralizzanti COVID-19

Metodo: ELISA Principio: Il reagente di rilevamento è realizzato con una tecnologia immunosoppressiva con anticorpi neutralizzanti. Il kit contiene il dominio di legame del recettore 2019-nCoV ricombinante marcato con perossidasi di rafano (RBD-HRP) e il recettore della cellula ospite ACE2 immobilizzato su piastra ELISA. Per informazioni scrivere a: [email protected] Manipolazione dei campioni Campioni: siero o [...]