ELISA kit for Rat TSH (Thyroid Stimulating Hormone)

A competitive Inhibition ELISA kit for detection of Thyroid Stimulating Hormone from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

A monoclonal antibody specific to Thyroid Stimulating Hormone (TSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Thyroid Stimulating Hormone (TSH) and unlabeled Thyroid Stimulating Hormone (TSH) (Standards or samples) with the pre-coated antibody specific to Thyroid Stimulating Hormone (TSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thyroid Stimulating Hormone (TSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Thyroid Stimulating Hormone (TSH) in the sample.

The Rat TSH ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.

• Species reactivity: Rat
• Sensitivity: 74.5pg/mL
• Detection range: 185.2-15000pg/mL
• Standard solution concentration: 15000pg/mL
• ELISA type: Competitive Inhibition
• Assay length: 2h

This kit is intended for research use only. Do not use for diagnostics purposes.