4-Hydroxynonenal Antibody, Clone 12F7: ATTO 488
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A monoclonal antibody from clone 12F7 against Human 4-Hydroxynonenal. The host species for the production of this antibody is Mouse. The antigen used for immunization is Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).. The antibody is tested and validated for WB, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:50); ELISA (1:1000). This MAb for 4-Hydroxynonenal is conjugated with ATTO 488.
- Target: 4-Hydroxynonenal
- Immunogen: Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
- Accession Number: N/A
- Gene ID: N/A
- Swiss-Prot: N/A
- Purification: Protein G Purified
- Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
- Specificity: Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
- References: 1. Peterson, D., Doorn, J. (2004) Free Radic Biol Med. 37(7):937-945.
- Storage: Conjugated antibodies should be stored at 4ºC
- Shipping: Blue Ice or 4ºC
- Conjugate: ATTO 488
- Clonality: Monoclonal
- Clone Number: 12F7
- Species Reactivity: Species Independent
- Host Species: MouseIsotype: IgG1
- Applications: WB, ICC/IF, ELISA
- Dilutions by application: WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
- Concentration: 1 mg/ml
This product is not for human or animal treatment or consumption. Not for use in diagnostics or therapeutics. For in vitro research use only.
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