TNFAIP3 Antibody

TNFAIP3 Antibody: TNFAIP3, also known as A20, is located in chromosome band 6q23, a region that is often deleted in B cell lymphomas. Recently, it was identified as a tumor suppressor gene in Hodgkin lymphoma and several subtypes of non-Hodgkin lymphomas. TNFAIP3 was initially identified as a zinc-finger protein that is rapidly and transiently induced by TNF-α, inhibiting NF-κB-dependent gene expression, and protecting cells from TNF-α-cytotoxicity. Overexpression of TNFAIP3 also inhibits the TLR2- and TLR4-mediated interleukin-8 synthesis in airway epithelial cells, suggesting that TNFAIP3 also acts as a negative regulator of TLR-mediated inflammatory responses, thereby protecting the host against harmful over-responses to pathogens. At least two isoforms of TNFAIP3 are known to exist.

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

• TNFAIP3 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Research area: Innate Immunity
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: N/A
• Immunogen: TNFAIP3 antibody was raised against a 17 amino acid synthetic peptide near the center of human TNFAIP3.
• The immunogen is located within amino acids 340 - 390 of TNFAIP3.
• Tested Applications: E, WB, IHC-P, IF
• Applications: TNFAIP3 antibody can be used for detection of TNFAIP3 by Western blot at 1 - 2 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1205 - Jurkat Cell Lysate
• Positive control 2: Cat. No. 10-101 - Human Lung Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 87 kDa
• Observed: 95 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: TNFAIP3 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: TNFAIP3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: Tnfaip3
• Accession #: Q60769
• Protein GI #: 2498097
• NCBI gene ID#: 21929
• NCBI official full name: tumor necrosis factor, alpha-induced protein 3
• NCBI organism: Mus musculus
• SWISSPROT #: Q60769

Optimal dilutions for each application to be determined by the researcher.