SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein

SARS-CoV-2 shares 79.5% sequence identity with SARS-CoV and is 96.2% identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. The coronaviral genome encodes four major structural proteins: the Spike (S) protein, Nucleocapsid (N) protein, Membrane/Matrix (M) protein and the Envelope (E) protein. The SARS Envelope (E) protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers, which may explain its role in viral budding and virion envelope morphogenesis. The SARS Membrane/Matrix (M) protein is one of the major structural viral proteins. It is an integral membrane protein involved in the budding of the viral particles and interacts with SARS Spike (S) protein and the Nucleocapsid (N) protein. The N protein contains two domains, both of them bind the virus RNA genome via different mechanisms.The CoV Spike (S) protein assembles as trimer and plays the most important role in viral attachment, fusion and entry. It is composed of a short intracellular tail, a transmembrane anchor and a large ectodomain that consists of a receptor binding S1 subunit (RBD domain) and a membrane-fusing S2 subunit. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor angiotensin-converting enzyme 2 (ACE2) present at the surface of epithelial cells.The SARS-CoV-2 Spike Protein S1 (RBD) (rec.) (His) is used as antigen in the Serological ELISA Kit to detect anti-SARS-CoV-2 Spike (RBD) antibodies in serum or plasma (see SARS-CoV-2 (Spike RBD) IgG Serological ELISA Kit; AG-45B-0020).

Short Term StorageL: +4°C, Long Term Storage: -20°C, Handling Advice: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. For maximum product recovery after thawing, centrifuge the vial before opening the cap. Use/Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

• Tested Applications: N/A
• Applications: N/A
• Predicted Molecular Weight: ~35kDa (SDS-PAGE)
• Physical state: Lyophilized
• Buffer: Lyophilized. Contains PBS.
• Concentration: 1mg/ml after reconstitution.
• NCBI official symbol: S
• Accession #: QHD43416.1
• Protein GI: N/A
• NCBI gene ID#: 43740568
• NCBI official full name: surface glycoprotein
• NCBI organism: Severe acute respiratory syndrome coronavirus 2
• Peptide sequence: Cys15-Gln1208 with mutation in the cleavage site (683 G)
• SWISSPROT #: P0DTC2
• Background Reference 1: N/A
• Background Reference 2: N/A
• Background Reference 3: N/A
• Background Reference 4: N/A
• Background Reference 5: N/A
• Source: HEK293 cells
• Species: Human
• By Source: Human Cells
• By Species: Human
• Fusion tag: N/A
• Sequence: Receptor-binding domain (RBD) of SARS-CoV-2 Spike protein S1 (aa 319-541) is fused at the C-terminus to a His-tag.
• Biology activity: Binds to anti-SARS-CoV-2 Spike (RBD) antibodies in serum or plasma.
• Purity: ≥95% (SDS-PAGE)

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