SARS-CoV-2 (COVID-19) Spike RBD Antibody

Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a Spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Coronavirus invades cells through Spike (S) glycoproteins, a class I fusion protein. It is the major viral surface protein that coronavirus uses to bind to the human cell surface receptor. It also mediates the fusion of host and viral cell membrane, allowing the virus to enter human cells and begin infection (3). The spike protein is the major target for neutralizing antibodies and vaccine development (4). The protein modeling suggests that there is strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19 (5). The recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein (6).

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

• SARS-CoV-2 (COVID-19) Spike RBD antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Research area: Infectious Disease, COVID-19
• Host species: Rabbit
• Species reactivity: Virus
• Homology: Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (63%)
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody (9087) was raised against a peptide corresponding to 19 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein RBD.
• The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19) Spike protein RBD.
• Tested Applications: E, WB, IF, IHC
• Applications: WB: 2-5 μ
• g/mL
• IF: 20 μ
• g/mL
• IHC: 0.5 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry and Immunofluorescence in human samples. SARS-CoV-2 (COVID-19) Spike RBD Antibody can be used for the detection of SARS-CoV-2 (COVID-19) Spike protein in ELISA and WB. It will detect 4 ng of free peptide at 1 μ
• g/mL. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: N/A
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: SARS-CoV-2 (COVID-19) Spike has one isoform (1273aa).
• Purification: SARS-CoV-2 (COVID-19) Spike RBD antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: SARS-CoV-2 (COVID-19) Spike RBD antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: S
• Accession #: QHD43416
• Protein GI #: 1791269090
• NCBI gene ID#: 43740568
• NCBI official full name: surface glycoprotein
• NCBI organism: Severe acute respiratory syndrome coronavirus 2
• SWISSPROT #: P0DTC2

Optimal dilutions for each application to be determined by the researcher.