SARS-CoV-2 (COVID-19) ORF3b Antibody
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Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF3b, ORF9b, ORF9c and ORF10. ORF3b may play a role in interferon antiviral system evasion (3).
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
- SARS-CoV-2 (COVID-19) ORF3b antibody can be stored at 4˚
- C for three months and -20˚
- C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Research area: Infectious Disease, COVID-19
- Host species: Rabbit
- Species reactivity: Virus
- Homology: Predicted reactivity based on immunogen sequence: SARS-CoV ORF3b: (92%).
- Immunogen: Anti-SARS-CoV-2 (COVID-19) ORF3b antibody (9281) was raised against a peptide corresponding to 13 amino acids near the amino-terminus of SARS-CoV-2 (COVID-19) ORF3b protein.
- The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF3b protein.
- Tested Applications: E, WB
- Applications: WB: 0.25 μ
- g/mL
- Antibody validated: SARS-CoV-2 (COVID-19) ORF3b antibody can detect 2 ng of free peptide at 1 μ
- g/mL in ELISA. It can detect SARS-CoV-2 ORF3b recombinant protein by ELISA and WB. All other applications and species not yet tested.
- Specificity: N/A
- Positive control 1: N/A
- Positive control 2: N/A
- Positive control 3: N/A
- Positive control 4: N/A
- Positive control 5: N/A
- Positive control 6: N/A
- Predicted molecular weight: 17kD
- MW explanation: N/A
- Validation: N/A
- Isoforms: N/A
- Purification: SARS-CoV-2 (COVID-19) ORF3b Antibody is affinity chromatography purified via peptide column.
- Clonality: Polyclonal
- Clone: N/A
- Isotype: IgG
- Conjugate: Unconjugated
- Physical state: Liquid
- Buffer: SARS-CoV-2 (COVID-19) ORF3b antibody is supplied in PBS containing 0.02% sodium azide.
- Concentration: 1 mg/mL
- NCBI official symbol: ORF3b
- Accession #: P0DTC3
- Protein GI #: 1835921851
- NCBI gene ID#: 43740569
- NCBI official full name: ORF3a protein
- NCBI organism: Severe acute respiratory syndrome coronavirus 2
- SWISSPROT #: P0DTC6
Optimal dilutions for each application to be determined by the researcher.
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