KappaB ras Antibody

KappaB ras Antibody: KappaB ras-1 (κB-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IκB (IκB), an inhibitor of the transcription factor NF-κB. IκB exists in two homologous forms, IκB-alpha and IκB-beta, although IκB-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive IκB-alpha-NF-κB complexes can shuttle in and out of the nucleus, IκB-beta-NF-κB complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the IκB-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of IκB-beta and regulating the rate of degradation of IκB-beta. This antibody detects both kappaB-ras1 and kappaB-ras2.

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

• KappaB ras antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Research area: Signal Transduction
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: Predicted species reactivity based on immunogen sequence: Chicken: (94%)
• Immunogen: KappaB ras antibody was raised against a 18 amino acid synthetic peptide from near the center of human KappaB ras 1.
• The immunogen is located within amino acids 80 - 130 of KappaB ras.
• Tested Applications: E, WB, IHC-P
• Applications: KappaB ras antibody can be used for detection of KappaB ras by Western blot at 2 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 1 μ
• g/mL.
• Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1283 - RAW 264.7 Cell Lysate
• Positive control 2: Cat. No. 1369 - Human Lymph node Tissue Lysate
• Positive control 3: Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: KappaB ras Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: KappaB ras Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• NCBI official symbol: NKIRAS1
• Accession #: AAF34998
• Protein GI #: 7008402
• NCBI gene ID#: 28512
• NCBI official full name: NFKB inhibitor interacting Ras-like 1
• NCBI organism: Homo sapiens
• SWISSPROT #: Q9NYS0

Optimal dilutions for each application to be determined by the researcher.