Spoligotyping Kit – tipizzazione di Mycobacterium tuberculosis

Alla vostra attenzione il nostro Spoligotyping Kit!

Questo kit per diagnostica molecolare è rapido e capace di identificare e tipizzare simultaneamente svariate tipologie del Complesso Mycobacterium tuberculosis da campioni clinici e campioni di cellule in coltura.

Uno strumento utile, economico ed efficace per la ricerca di Tubercolosi/ Mycobacterium. Spoligotyping è basato sull’uso di PCR per la ricerca e tipizzazione delle specie appartenenti al Complesso di Mycobacterium tuberculosis. Spoligotyping, che utilizza la tecnica RLB (Reversed Line Blotting) offre un’alternativa rispetto al classico Southern blotting nel caso siano neccessari risultati in breve tempo. Il metodo è particolarmente utile per ricercare e simultaneamente tipizzare M. tuberculosis in campioni clinici (sospette infezioni nosocomiali, infezioni e focolai nelle prigioni, ecc.). Il livello di differenziazione attraverso spoligotyping è inferiore se confrontato con il fingerprinting di IS6110 per ceppi che hanno cinque o più copie di IS6110, ma è più elevato per ceppi con meno di cinque copie. Per questa ragione, Spoligotyping è un metodo di tipizzazione preferito verso ceppi di M. bovis, che solitamente contiene una o al massimo due copie di IS6110.

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  • Spoligotyping Membrane
    A very simple, inexpensive and effective tool for Tuberculosis/ Mycobacterium research. Spoligotyping is a PCR-based Method to Simultaneously Detect and Type Mycobacterium Tuberculosis Complex Bacteria. Spoligotyping, which uses RLB (Reversed Line Blotting) offers an alternative for typical Southern blotting when rapid results are required. The method is particularly useful to simultaneously detect and type M. tuberculosis complex bacteria in clinical samples (suspected nosocomial infections, outbreaks in prisons, etc.). The level of differentiation by spoligotyping is less compared to IS6110 fingerprinting for strains having five or more IS6110 copies, but higher for strains with less than five copies. Thus, Spoligotyping is a preferred method to type M. bovis strains, which usually contain only one or two IS6110 copies. Note, that Mycobacterium bovis can be recognized by the absence of reactivity with spacers 39-43.
    IM9702 1536.00 EUR

  • Spoligotyping Kit with Primers and Controls
    A very simple, inexpensive and effective tool for Tuberculosis/ Mycobacterium research. Spoligotyping is a PCR-based Method to Simultaneously Detect and Type Mycobacterium Tuberculosis Complex Bacteria. Spoligotyping, which uses RLB (Reversed Line Blotting) offers an alternative for typical Southern blotting when rapid results are required. The method is particularly useful to simultaneously detect and type M. tuberculosis complex bacteria in clinical samples (suspected nosocomial infections, outbreaks in prisons, etc.). The level of differentiation by spoligotyping is less compared to IS6110 fingerprinting for strains having five or more IS6110 copies, but higher for strains with less than five copies. Thus, Spoligotyping is a preferred method to type M. bovis strains, which usually contain only one or two IS6110 copies. Note, that Mycobacterium bovis can be recognized by the absence of reactivity with spacers 39-43.
    IM9701 1620.00 EUR

  • Mini-blotter for Spoligotyping
    This is mini-blotter unit only. Spoligotyping Kit with primers and controls IM9701 is not included.
    MN45 3828.00 EUR

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