Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit

Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

For use with PE5700, MJ-Opticon & other single color systems, ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycler iQ4, iCycler iQ5 & other multi-color systems

• Shipping: dry ice
• All reagents should be stored at -20°C. Storage at +4°C is not recommended.

Analysis sensitivity: 1×10^3 copies/ml

On January 11, 2020, Chinese health authorities preliminarily identified more than 40 human infections with a novel coronavirus in an outbreak of pneumonia under investigation in Wuhan City, Hubei Province, China.The Chinese authorities identified a new type of coronavirus (novel coronavirus, named as 2019-nCoV), which was isolated on 7 January 2020.Coronaviruses are a large family of viruses, some causing illness in human and others circulating among animals such as camels, cats and bats. 2019-nCoV is a novel coronavirus. The primer andprobe design for this kit is based on the newly released strain (2019-nCoV) (GeneBank accession: MN908947) and covers 6 2019-nCoV strains sequences (EPI_ISL_402119, EPI_ISL_402120, EPI_ISL_402121, EPI_ISL_402122, EPI_ISL_402123, EPI_ISL_402124 included).Thekit contains a specific ready-to-use system for the detection of NovelCoronavirus (2019-nCoV) by Reversetranscription Polymerase Chain Reaction(RT-PCR) in the real-time PCR system. The master contains a Super Mix for the specific amplification ofvirusRNA. The reaction is done in one step real time RT-PCR. The first step is a reverse transcription (RT), during which the virusRNA is transcribed into cDNA. Afterwards, a thermostable DNA polymerase is used to amplify the specific gene fragments by means of polymerase chain reaction (PCR). Fluorescence is emitted and measured by the real time systems ́ optical unit during PCR. The detection of amplified virusDNA fragment is performed in fluorimeterchannelFAMand channel HEX/VIC/JOEwith the fluorescent quencher BHQ1. In addition, the kit contains a systemto identify possible PCR inhibition by measuring the CalRed 610/ROX/TEXAS RED fluorescence of the internal control (IC).