ELISA kit for General cGMP (Cyclic Guanosine Monophosphate)
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A competitive Inhibition ELISA kit for detection of Cyclic Guanosine Monophosphate from General in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
A monoclonal antibody specific to Cyclic Guanosine Monophosphate (cGMP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cyclic Guanosine Monophosphate (cGMP) and unlabeled Cyclic Guanosine Monophosphate (cGMP) (Standards or samples) with the pre-coated antibody specific to Cyclic Guanosine Monophosphate (cGMP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cyclic Guanosine Monophosphate (cGMP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cyclic Guanosine Monophosphate (cGMP) in the sample.
The General cGMP ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
- Species reactivity: General
- Sensitivity: 45.2pg/mL
- Detection range: 123.5-10000pg/mL
- Standard solution concentration: 10000pg/mL
- ELISA type: Competitive Inhibition
- Assay length: 2h
This kit is intended for research use only. Do not use for diagnostics purposes.
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