Recombinant 2019-nCoV coronavirus Spike protein, S1 subunit, expressed in Baculovirus-Insect cells
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Recombinant COVID-19 (2019 novel coronavirus) Spike protein S1 subunit was fused to His-tag at C-terminus and expressed in Baculovirus-Insect cells. The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell.S1 mainly contains a receptor binding domain (RBD) and recognize the cell surface receptor. S2 essential for membrane fusion. S protein are important for neutralizing-antibody and T-cell responses, as well as protective immunity.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at +4 degrees Celsius before opening to recover the entire contents.
- Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
- Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
- Expression system:Baculovirus-Insect Cells
- Origin species: 2019-nCoV
- Tag: His
- Predicted N Terminal: Val
- Endotoxin: <1.0 EU per μg protein as determined by the LAL method
- Purity: >90% as determined by SDS-PAGE
- Protein length: 681 AA
- Form: Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization
- Molecular Mass: The recombinant COVID-19 spike protein S1 consists of 681 amino acids and predicts a molecular mass of 76,45 kDa.
For research use only, not to be used for human or animal diagnostics or treatment.
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